Muller Kauffmann Tetrathionate Broth with Novobiocin (MKTTn)

Tetrathionate broth for the enrichment of Salmonellae from food samples according to the ISO 65 79 standard.

Meat extract 4,3
Peptone from casein 8,6
Sodium chloride 2,6
Calcium carbonate 38,7
Sodium thiosulfate, dehydrated 30,5
Ox bile, dehydrated 4,78
Brilliant green 0,0096
Novobiocin 0,04
Final pH 8.2 ± 0.2 at 25 °C.

Tetrathionate broth for the selective enrichment of Salmonellae from food samples in compliance with the ISO 6579 standard.
DIRECTIONS
Suspend 89.5 g in 1 litre of demineralised water. Bring to the boil for 5 minutes.
Do not autoclave.
Cool to 45°C and add to 1 litre of medium:
- 20 ml of a solution containing 5 g of potassium iodide and 4 g of iodine.
INCUBATION
Suspend 1 ml of pre-enrichment medium in 10 ml of MKTTn. Incubate 21-27 hours at 36-38°C.

145.005878.54 Merck 5878 500 g