Meat Liver Agar

For the enumeration of sulphite-reducing clostridia in foods.

See also?Anaerobiosis Jar?

Ready-to-Use product:
- Storage: 2-8°C in the dark

See also:
- Anaerocult.
- Anaerotest.
- Anaerobiosis jar.
- Ready-to-use.

Meat-Liver Base (Biokar,
Diagnostic Pasteur) 30.0
(Merck) 20.0
Glucose (Biokar) 1.0
(Merck) 0.75
(Diagnostic Pasteur) 2.0
Sodium sulphite (Biokar) 0.5
(Merck) 1.2
(Diagnostic Pasteur) 1.0
Ammonium iron (III) citrate 0.5
Soluble starch (Biokar) 1.0
(Merck) 0.75
(Diagnostic Pasteur) 2.0
Sodium carbonate (Merck) 0.67
Agar-agar (Biokar) 8.0
(Merck, Diagnostic Pasteur) 11.0
Final pH 7.5 ± 0.2 (Diagnostic Pasteur)
Final pH 7.4 ± 0.2 (Biokar)
Final pH 7.6 ± 0.2 (Merck)

For the enumeration of sulphite-reducing clostridia spores in foods.
Suspend 41 g/l (Biokar), 34 g/l (Merck), 46.5 g/l (Diagnostic Pasteur).Autoclave for 20 minutes at 115°C. Cool to 50°C.
Inoculate 1 ml of sample material which has been pre-warmed to destroy vegetative cells and activate spores.
Incubate anaerobically 24 h at 37°C.
READING
? Sulphite-reducing microorganisms appear black.