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For the confirmation of faecal streptococci in water after culture in Rothe medium.
Suspend 35.7 g/l. Autoclave 15 minutes at 121°C. The medium is inoculated from a culture on Rothe medium.
Incubation 24-48 hours at 37°C.
Peptone 20,0
Glucose 5,0
Sodium chloride 5,0
Potassium dihydrogen phosphate 2,7
Dipotassium phosphate 2,7
Sodium azide 0,3
Ethyl violet 0,0005
pH final 6,8 ± 0,2