Listeria Selective Enrichment Broth UVM-I

For the detection of Listeria monocytogenes

See also
ÒSupplement UVM-II for Listeria Selective Broth UVM-I Merck 4039, Oxford Agar
and Palcam agarÓ

PRE-ENRICHMENT
UVM-I
and
ENRICHMENT
UVM-II
FOR THE DETECTION OF LISTERIA IN MEAT
AND MEAT PRODUCTS

Tryptose 10.0
Yeast extract 5.0
Meat extract 5.0
Esculin 1.0
Sodium chloride 20.0
Disodium hydrogen phosphate 12.0
Potassium dihydrogen phosphate 1.35
Nalidixic acid 0.02
Acriflavine hydrochloride 0.012
Final pH 7.2 ± 0.2 at 25°C

For the detection of Listeria monocytogenes in meat and meat products it is advisable to carry out a pre-enrichment in UVM-I broth followed by an enrichment in UVM-II broth.
Preparation of pre-enrichment broth UVM-I:
- Suspend 54.4 g/l distilled water.
- Autoclave 121°C/15 min.
Preparation pre-enrichment broth UVM-II:
- Add one vial of UVM-II Supplement Merck 4039 to 1 litre of pre-enrichment broth UVM-I cooled to 45°C.