Lactobacilli MRS Agar

For the enumeration of Lactobacilli

Universal Peptone 10.0
Yeast extract 5.0
Meat extract (Merck) 5.0
(Diagnostic Pasteur) (Biokar) 10.0
Glucose 20.0
Dipotassium hydrogen phosphate 2.0
Sodium acetate 5.0
Ammonium citrate 2.0
Magnesium sulphate
(Diagnostic Pasteur) (Merck) 0.1
(Biokar) 0.2
Manganese sulphate 0.05
Tween 80 1.08
Agar (Merck)12.0
(Biokar) 15.0
(Diagnostic Pasteur) 13.0
Final pH 6.5 ± 0.2 at 25°C.

For the enumeration of Lactobacilli in dairy and other food products.
Suspend 70.3 g/l (Biokar) or 66.2 g/ l (Merck) or 68 g/l (Diagnostic Pasteur).Autoclave 15 minutes at 120°C.
Incubate in anaerobiosis jar 2-3 days at 37°C.

112.064247.54 Diagnostic Pasteur 64244 500 g
145.010660.54 Merck 10660 500 g
250.033821.54 Biokar 089 500 g
747.023270.02 ATL® , MRS broth Ready-to-use 100 X 9 ml tubes