Fraser Broth Base

Pre-enrichment and enrichment broth for the detection of Listeria in food products.

See also ÒListeria ChromagarÓ

Proteose peptone 5.0
Peptone from casein 5.0
Meat extract 5.0
Yeast extract 5.0
Sodium chloride 20.0
Disodium hydrogen phosphate 10 H2O 12.0
Potassium dihydrogen phosphate 1.35
Esculin 1.0
Lithium chloride 3.0
pH control 7.2 ± 0.2 at 25°C

Pre-enrichment and enrichment broth for the detection of Listeria in food products.
Directions:
Suspend 57.4 g in 1 litre of demineralised water.
Autoclave at 121°C/15 min.
For the half-concentrated Fraser broth: add the reconstituted contents of one vial of antibiotic mixture and one vial of ammonium iron (III) citrate into 1 litre of broth cooled to 50°C.
For the Fraser broth: add the reconstituted contents of a second vial of antibiotic mixture.
See also ÒSelective supplement for Fraser broth base Merck 10399Ó.

145.010398.54 Merck 10398 500 g
157.009110.02 ATL¨ stainless steel stand for ready-to-use broth bag
747.021175.02 ATL¨ , Fraser Ready-to-use 100 X 10-ml tube pack
747.033170.02 ATL¨, Fraser 1/2 Ready-to-use 10 X 225 ml vials
747.046170.02 ATL¨, Fraser 1/2 Ready-to-use 4 X 3-litre bag pack
747.047170.02 ATL¨, Fraser 1/2 Ready-to-use 2 X 5-litre bag pack
770.000108.02 Connector for ready-to-use broth bag