E. coli test + Kovacs Reagent

E. coli identification

2 - Add one drop of Kovacs reagent in the cuvette.A red colour 1-2 minutes later indicates the presence of tryptophanase.See also ?UV Light?----------Test strips 50Cuvettes 50Tray 1Kovacs reagent 1 x 5 ml----------E. coli identification takes place through the detection of the enzymes ß-D-Glucuronidase and tryptophanase (indol production) :- 94% of E. coli possess ß-D-Glucuronidase.- 99% of E. coli possess tryptophanase.TECHNIQUE1 - Remove a colony with a platinum loop, suspend it 200 µl distilled water in a reaction cuvette.Placer la bandelette dans cette cuvette.Incubate at 37° C for 30-120 min.Evaluate with a UV lamp blue fluorescence indicates the presence of ß-D-Glucuronidase.----------See also?ECC Agar?----------

145.013303.02 Merck 13303 50 test pack