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Pick off the colony to be tested with a platinum loop and spread it on a dry glass slide. Add a drop of reagent. Immediate oxygen release is indicative of a positive reaction. The reagent may also be applied onto colonies grown on solid blood-free medium.
Biochemical test to confirm catalase enzyme activity.
Catalytic activity is present in all aerobic cells.Presence or absence of catalytic activity is a taxonomic characteristic of microorganisms.
It is therefore used to differentiate and identify microorganisms.
Hydrogen peroxide 3%