E. coli 0157 Agar

E. Coli 0157 detection

See also
Singlepath
and E. coli
0157 latex

DIRECTIONS
? For a 1000 ml package:
- Prepare 1000 ml of demineralised or distilled water for a 100 test dose.
- Suspend the powdered medium in the water, swirl to mix and allow to swell.
- Bring gently to the boil, stirring until completely dissolved.
- Cool to 47° C ± 2°C and keep the CHROMagar® E. coli 0157 Agar molten at this temperature.
? For a 5 litre package:
- Suspend 29 g/l demineralised water.
- Bring to the boil, stirring until completely dissolved, then proceed as above.

INNOVATIVE
The CHROMagar® E. coli 0157 Agar uses a selective chromogenic mixture compatible with conventional agar surface culture (surface spread technique).
(NF V08-032 compliant)

(NF V08-032 compliant)
SIMPLIFIED
E. coli 0157
DETECTION
?
• Straightforward reading of
violet-coloured
E. coli 0157 and
metallic blue-coloured
E. coli and Coliforms

PrincipLe
? Incubate the sample in mTSB enrichement broth. Following separation and concentration using immunomagnetic beads, surface-inoculate the sample on CHROMagar® E. coli 0157 Agar and Sorbitol-Mac Conkey Agar (SMAC Agar).
(NF V08-032 standard-compliant)
? Innovative culture medium designed by A. Rambach for the detection and enumeration of:
- E. coli 0157; through a simple count of violet-coloured colonies…
- E. coli; forming bleue-coloured colonies…
… thanks to an specific marker.
BENEFITS
? Simple
Thanks to the use of a staining method compatible with the surface spread technique, the CHROMagar® E. coli 0157 Agar allows for naked eye reading under normal laboratory lighting through a simple count of violet colonies.
? Reliable
An efficient and more accurate colony count is obtained thanks to the selective chromogenic mixture.
See also: «ECC Agar»,«TBX Agar», «Rapid E. coli 2», «Chromocult Coliform Agar»

Selective chromogenic mixture 1.0
Peptone and salt 13.0
Agar-agar 15.0
Final pH 6.8 ± 0.2 at 25°C.

E. Coli 0157 Detection

VERY
SELECTIVE

FAST - simplE - spECIFIC - COST-EFFICIENT
(NF V08-032 compliant)