ECC Agar

For the detection and enumeration of blue-coloured E. coli and red-coloured Coliforms.

DIRECTIONS
Prepare 1000 ml of distilled or demineralised water for a 100 test dose.
Suspend powdered medium in the water, stir to mix and allow to swell.
Bring gently to the boil, stirring until completely dissolved.
Cool to 47±2°C and keep the CHROMagar® ECC Agar molten at this temperature.
PROCEDURE
Homogenise sample if required. Inoculate 1 ml into 10 ml of ECC Agar CHROMagar®.
Incubate at 37°C/24 h.
Count the caracteristic colonies:
• Red colonies: Coliforms
• Blue colonies: E. coli
HUMEAU Suggestion
Incubate at 44°C/24 h.
Count the caracteristic colonies:
• Blue colonies: E. coli
• Red + blue colonies=
total faecal Coliforms

INNOVATIVE
The ECC Agar is an innovative culture medium designed by A. Rambach for the detection of blue-appearing E. coli and red-appearing Coliforms thanks to a specific marker. The rest of Gram-negative bacteria grow as colourless colonies.

SIMULTANEOUS
AND
SIMPLIFIED DETECTION
of E. coli
and Coliforms
TRANSPARENT
AGAR:
• Straightforward reading of
blue E. coli
and
red Coliforms

BENEFITS
Time-saving
Ease of use (pour-plate technique)
Transparent agar makes reading easier
Reliability: the selective chromogenic mixture provides an efficient and more accurate colony count.

Pour-plate technique

Surface spread technique