E. coli Agar

Detection and enumeration of blue-coloured E. coli using the colony count technique.

DIRECTIONS
? Prepare 1000 ml demineralised or distilled water for a 100 test dose.
? Suspend the powdered medium in the water, stir to mix and allow to swell.
? Bring gently to the boil, stirring until completely dissolved.
? Cool to 47±2°C and keep the E. coli Agar CHROMagar® molten at this temperature.
PROCEDURE
? Homogenise the sample if required. Inoculate 1 ml into 10 ml of CHROMagar® E. coli Agar. (final pH 6.0±0.2). Incubate at 44°C for 18-24 h.
Count the blue colonies.

INNOVATIVE-
CHROMagar® E. coli Agar contains a selective chromogenic mix which may be used in combination with the pour-plate method.

DESCRIPTION
? CHROMagar® E. coli Agar is an innovative culture medium designed by A. Rambach for the detection and enumeration of blue-coloured E. coli with a simple colony count:
• E. coli colonies develop a blue colour due to the presence of a specific marker in the medium
• Gram-negative bacteria grow as colourless or white colonies,
• Gram-positive bacteria are inhibited.
BENEFITS
? Ease of use
CHROMagar® E. coli Agar allows for naked eye reading in normal laboratory lighting by performing a simple count of blue colonies. It uses a staining technique compatible with the pour-plate method. The transparent agar facilitates reading and eliminates the need for special training.
? Accuracy
An efficient and more accurate colony count is obtained thanks to the selective chromogenic mixture.

Selective chromogenic mix 9.0
Yeast and meat extract 3.0
Peptone 5.0
Sodium chloride 5.0
Agar-agar 15.0
Final pH 6.0 ± 0.2

E. Coli detection

VERY SELECTIVE